Objective: The paper explores the impact of DRG payment reform on the hospitalization costs of cerebral stroke rehabilitation cases. Method: The influence of DRG payment reform on the hospitalization cost of cerebral stroke rehabilitation cases was discussed by comparing and analyzing the general situation, hospitalization days, hospitalization cost and composition of hospitalization cost of cerebral stroke rehabilitation cases in a specialized hospital in Nanning, Guangxi Zhuang Autonomous Region before and after the reform. Result: The results showed that after the implementation of DRG payment reform, the hospitalization days of cerebral stroke rehabilitation patients decreased from 28 days to 21 days (Z=-18.569, P<0.001). The median hospitalization costs of cerebral stroke rehabilitation decreased from 25303.44 yuan to 22512.44 yuan after implementation (Z=-9.616, P<0.001), and the difference is statistically significant. The decrease in the subcategories of hospitalization costs was also observed. The proportion of hospitalization costs for rehabilitation, diagnosis and treatment and comprehensive services increased, while the proportion of hospitalization costs for drugs and consumables decreased. Conclusion: The DRG payment reform will reduce the hospitalization cost and shorten the hospitalization time of cerebral stroke rehabilitation cases, but it is not suitable for cerebral stroke rehabilitation cases. It is recommended that the medical insurance department should actively investigate the needs of rehabilitation cases and explore the application of bed-day payment and bed-day-point payment for rehabilitation cases based on local situations. Medical institutions should improve the homogeneity of rehabilitation treatment, ensure the effective treatment of rehabilitation patients and the reasonable use of medical insurance funds.