Objective: The paper investigates the education and professional background of health insurance workforces, analyzes the awareness and influencing factors of health insurance workforces on the health insurance payment reform policy, and puts forward suggestions for improving the management ability of health insurance workforces. Methods: In 2021, practitioners from different regions of the medical insurance sector were selected to conduct an online questionnaire survey and collect data, mainly including demographic characteristics, knowledge of medical insurance payment methods, and work commitment. SPSS23.0 statistical software was used for rank sum test, Spearman correlation analysis and disordered multinomial logistic regression analysis. Results: A total of 280 valid questionnaires were submitted. Results: Among the respondents, 55.7% were under the age of 40, 68.6% had a bachelor's degree or above, 65.4% had 10 years' work experience, 7.9% had a professional background in insurance, and 44.3% had a medical and nursing background. The results of logistic regression analysis show that the factors affecting medical insurance practitioners' knowledge of medical insurance payment methods include the working years, the job title, the work organization, and the region. Conclusion: The training and continuing education of health insurance workforces should be strengthened, a professional title promotion system should be established, a professional medical insurance talent team should be built, and the efficient operation of the reform of medical insurance payment methods should be promoted.