Objective: The paper is to analyze the nursing effect of multidisciplinary cooperation in cholecystectomy and its impact on patients' out-of-pocket expenses. Methods: 84 patients with cholecystectomy in our hospital from December 2021 to April 2022 were randomly divided into 42 patients in the control group receiving routine nursing and 42 patients in the observation group receiving multidisciplinary cooperation. After 14 days of nursing, the nursing effect and cost was evaluated, and the social support, medical coping style, out-of-pocket expenses and satisfaction of the two groups were compared. Results: The scores of objective support, subjective support and utilization of support in the observation group were higher than those in the control group (P﹤0.05), the face-to-face and satisfaction scores were higher than those in the control group (P﹤0.05), and the scores of avoidance, submission and self payment were lower than those in the control group (P﹤0.05). Conclusion: The effect of multidisciplinary collaboration on patients with cholecystectomy can reduce out-of-pocket expenses, improve social support for patients, improve medical coping style and improve nursing satisfaction, which is worthy of promotion and application.